Comparison of the Phenotyping Methods ID 32E and VITEK 2 Compact GN with 16S rRNA Gene Sequencing for the Identification of Enterobacter sakazakii-Reference-Cited by-同舟云学术

Comparison of the Phenotyping Methods ID 32E and VITEK 2 Compact GN with 16S rRNA Gene Sequencing for the Identification of Enterobacter sakazakii

Published:2007-06 Issue:6 Volume:45 Page:2048-2050
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Fanjat Nadège¹,Leclercq Alexandre²,Joosten Han³,Robichon Denis¹

Affiliation:

1. bioMérieux, Research and Development Microbiology, La Balme Les Grottes, France

2. Institut Pasteur, Microbiology Department, 28 rue du Dr Roux, 75724 Paris cedex 15, France

3. Nestle Research Centre, Quality and Safety Department, CH-1000 Lausanne 26, Switzerland

Abstract

ABSTRACT A total of 34 isolates (28 Enterobacter sakazakii and 6 Enterobacteriaceae ) from infant formulae, milk powder, and the production environment of milk powder factories were identified using ID 32E and VITEK 2 compact GN systems (bioMérieux, France). The ID 32E version 3.0 and VITEK 2 compact GN version 01.01b correctly identified 100% (28) of the Enterobacter sakazakii isolates tested, whereas the previous software version 2.0 for ID 32E showed only 71.4% correct results. None of the non- E. sakazakii isolates tested were misidentified as E. sakazakii with either of the identification systems used.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00961-06

Reference21 articles.

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