Affiliation:
1. School of Life Science and Biotechnology, Dalian University of Technology, Dalian, People's Republic of China
2. School of Life Sciences, Tsinghua University, Beijing, People's Republic of China
Abstract
In the present study, we have addressed efficient, time-saving, and cost-effective CRISPR-based phage genome editing of
Klebsiella
phage, which has the potential to significantly expand our knowledge of phage-host interactions and to promote applications of phage therapy. The distribution of sgRNA activity was first evaluated in phages. Short homologous arms were proven to be enough to introduce point mutation, small frameshift deletion, gene deletion, and swap into phages, and weak sgRNAs were proven useful for precise phage genome editing but failed to select random recombinants, all of which makes the CRISPR-based phage genome-editing method easier to use.
Funder
National Natural Science Foundation of China
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Cited by
38 articles.
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