Cooccurrence of
ScDSP
Gene Expression, Cell Death, and DNA Fragmentation in a Marine Diatom,
Skeletonema costatum
-
Published:2005-12
Issue:12
Volume:71
Page:8744-8751
-
ISSN:0099-2240
-
Container-title:Applied and Environmental Microbiology
-
language:en
-
Short-container-title:Appl Environ Microbiol
Author:
Chung Chih-Ching1, Hwang Sheng-Ping L.2, Chang Jeng1
Affiliation:
1. Institute of Marine Biology, National Taiwan Ocean University, Keelung 20224, Taiwan, Republic of China 2. Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan, Republic of China
Abstract
ABSTRACT
A novel death-specific gene,
ScDSP
, was obtained from a death stage subtraction cDNA library of the diatom
Skeletonema costatum
. The full length of
ScDSP
cDNA was 921 bp in length, containing a 699-bp open reading frame encoding 232 amino acids and two stretches of 66 and 156 bp in the 5′ and 3′ untranslated regions, respectively. Analysis of the peptide structure revealed that ScDSP contained a signal peptide domain, a transmembrane domain, and a pair of EF-hand motifs. When
S. costatum
grew exponentially at a rate of 1.3 day
−1
, the
ScDSP
mRNA level was at 2 μmol · mole 18S rRNA
−1
. In contrast, when the culture entered the death phase with a growth rate decreasing to 0.5 day
−1
,
ScDSP
mRNA increased dramatically to 668 μmol · mole 18S rRNA
−1
, and a high degree of DNA fragmentation was simultaneously observed. Under the influence of a light-dark cycle,
ScDSP
expression in both exponential and stationary phases clearly showed a diel rhythm, but the daily mean mRNA level was significantly higher in the stationary phase. Our results suggest that
ScDSP
may play a role in the molecular mechanism of self-destructive autolysis in phytoplankton under stress.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference44 articles.
1. Agustí, S., M. P. Satta, M. P. Mura, and E. Benavent. 1998. Dissolved esterase activity as a tracer of phytoplankton lysis rates in the northwestern Mediterranean. Limnol. Oceanogr.43:1836-1849. 2. Armbrust, E. V., J. A. Berges, C. Bowler, B. R. Green, D. Martinez, N. H. Putnam, S. Zhou, A. E. Allen, K. E. Apt, M. Bechner, M. A. Brzezinski, B. K. Chaal, A. Chiovitti, A. K. Davis, M. S. Demarest, J. C. Detter, T. Glavina, D. Goodstein, M. Z. Hadi, U. Hellsten, M. Hildebrand, B. D. Jenkins, J. Jurka, V. V. Kapitonov, N. Kröger, W. W. Y. Lau, T. W. Lane, F. W. Larimer, J. C. Lippmeier, S. Lucas, M. Medina, A. Montsant, M. Obornik, M. S. Parker, B. Palenik, G. J. Pazour, P. M. Richardson, T. A. Rynearson, M. A. Saito, D. C. Schwartz, K. Thamatrakoln, K. Valentin, A. Vardi, F. P. Wilkerson, and D. S. Rokhsar. 2004. The genome of the diatom, Thalassiosira pseudonana: ecology, evolution, and metabolism. Science306:79-86. 3. The Ecological Role of Water-Column Microbes in the Sea 4. Berges, J. A., and P. G. Falkowski. 1998. Physiological stress and cell death in marine phytoplankton: induction of proteases in response to nitrogen or light limitation. Limnol. Oceanogr.43:129-135. 5. Berridge, M. J., P. Lipp, and M. D. Bootman. 2000. The versatility and universality of calcium signalling. Nat. Rev. Mol. Cell Biol.1:11-21.
Cited by
44 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|