Affiliation:
1. Department of Immunology, School of Biological Sciences, Madurai Kamaraj University, India.
Abstract
Investigations were undertaken to characterize the protective immunity induced by porin-lipopolysaccharide (LPS) against Salmonella typhimurium infection in mice. Mice immunized with porin-LPS showed higher levels of antiporin immunoglobulin G than mice which received porin alone. Further, T cells from porin-LPS-immunized mice showed an augmented proliferative response to porin in vitro compared with the response of T cells from porin-injected animals. The passive transfer of anti-LPS antibodies conferred significant protection (17%), while antiporin serum failed to protect mice against lethal challenge, indicating the protective ability of anti-LPS antibodies. However, the transfer of serum obtained from porin-LPS-immunized mice resulted in better protection (30%) than did anti-LPS or antiporin antibodies alone. In contrast to LPS, monophosphoryl lipid A completely failed to induce protection against lethal infection. However, comparable to the effect of LPS, injection of porin with monophosphoryl lipid A enhanced antibody response and the protective ability of porin (81.25%). The transfer of T cells from porin-LPS-immunized mice provided higher levels of protection (47%) against lethal challenge than did T cells from porin-immunized mice (23%). The combination of T cells and serum from porin-immunized mice transferred 36% protection. However, a combination of T cells and serum from porin-LPS-immunized mice conferred the highest level of protection (92%), which was reflected by the number of survivors (100%) in the porin-LPS-immunized group. These results demonstrate that besides the protective effect of anti-LPS antibodies, the ability of LPS to augment humoral and cell-mediated immune responses to porin confers effective protection against Salmonella infection.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference53 articles.
1. Requirement of thymus-dependent Iymphocytes for potentiation by adjuvants of antibody synthesis;Allison A. C.;Nature (London),1971
2. Activation of T and B lymphocytes in vitro. I. Regulatory influence of bacterial lipopolysaccharide (LPS) on specific T-cell helper function;Armerding D.;J. Exp. Med.,1974
3. Proteins from Salmonella R. mutants mediating protection against Salmonella typhimuium infection in mice. I. Preparation of proteins free from lipopolysaccharide using various chromatographic methods. Zentralbl;Bhatnagar N.;Bakteriol. Hyg. 1 Abt. Orig. A,1982
4. LPS and specific T-cell responses: interleukin/(IL1)-independent amplification of antigen-specific T helper (TH) cell proliferation;Bismuth G.;J. Immunol.,1985
5. Cell mediated immunity to bacterial infection in the mouse: thymus derived cells as effectors of acquired resistance to Listeria monocytogenes;Blanden R. V.;Scand. J. Immunol.,1972
Cited by
32 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献