Recognition of Potentially Novel Human Disease-Associated Pathogens by Implementation of Systematic 16S rRNA Gene Sequencing in the Diagnostic Laboratory-Reference-Cited by-同舟云学术

Recognition of Potentially Novel Human Disease-Associated Pathogens by Implementation of Systematic 16S rRNA Gene Sequencing in the Diagnostic Laboratory

Published:2010-09 Issue:9 Volume:48 Page:3397-3402
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Keller Peter M.¹,Rampini Silvana K.²,Büchler Andrea C.³,Eich Gerhard⁴,Wanner Roger M.⁵,Speck Roberto F.³,Böttger Erik C.¹,Bloemberg Guido V.¹

Affiliation:

1. University of Zurich, Institute of Medical Microbiology, Gloriastrasse 30/32, CH-8006 Zurich, Switzerland

2. Division of Internal Medicine, University Hospital Zurich, Raemistrasse 100, CH-8091 Zurich, Switzerland

3. Division of Infectious Diseases and Hospital Epidemiology, University of Zurich, University Hospital of Zurich, Raemistrasse 100, CH-8091 Zurich, Switzerland

4. Division of Infectious Diseases and Hospital Hygiene, Department of Internal Medicine, Stadtspital Triemli, CH-8063 Zurich, Switzerland

5. Department of Internal Medicine, Kantonsspital Winterthur, CH-8401 Winterthur, Switzerland

Abstract

ABSTRACT Clinical isolates that are difficult to identify by conventional means form a valuable source of novel human pathogens. We report on a 5-year study based on systematic 16S rRNA gene sequence analysis. We found 60 previously unknown 16S rRNA sequences corresponding to potentially novel bacterial taxa. For 30 of 60 isolates, clinical relevance was evaluated; 18 of the 30 isolates analyzed were considered to be associated with human disease.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01098-10

Reference32 articles.

1. Comparison of Conventional and Molecular Methods for Identification of Aerobic Catalase-Negative Gram-Positive Cocci in the Clinical Laboratory

2. Ribosomal DNA Sequencing for Identification of Aerobic Gram-Positive Rods in the Clinical Laboratory (an 18-Month Evaluation)

3. 16S rRNA Gene Sequencing versus the API 20 NE System and the VITEK 2 ID-GNB Card for Identification of Nonfermenting Gram-Negative Bacteria in the Clinical Laboratory

4. Böttger, E. C. 1996. Approaches for identification of microorganisms. ASM News 62 : 247-250.

5. Böttger, E. C. 1989. Rapid determination of bacterial ribosomal RNA sequences by direct sequencing of enzymatically amplified DNA. FEMS Microbiol. Lett. 53 : 171-176.

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