Comparison of Multiple-Locus Variable-Number Tandem-Repeat Analysis with Other PCR-Based Methods for Typing Brucella suis Isolates-Reference-Cited by-同舟云学术

Comparison of Multiple-Locus Variable-Number Tandem-Repeat Analysis with Other PCR-Based Methods for Typing Brucella suis Isolates

Published:2007-12 Issue:12 Volume:45 Page:4070-4072
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

García-Yoldi David¹,Le Fleche Philippe²³,De Miguel María J.⁴,Muñoz Pilar M.⁴,Blasco José M.⁴,Cvetnic Zeljko⁵,Marín Clara M.⁴,Vergnaud Gilles²³,López-Goñi Ignacio¹

Affiliation:

1. Departamento de Microbiología y Parasitología, Universidad de Navarra, 31008 Pamplona, Spain

2. Université Paris-Sud, Institut de Génétique et Microbiologie, Orsay F-91405, and CNRS, Orsay F-91405, France

3. Centre d'Etudes du Bouchet, 5 rue Lavoisier, 91710 Vert le Petit, France

4. Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Gobierno de Aragón, 58080 Zaragoza, Spain

5. Croatian Veterinary Institute, Department of Immunology, Savska Cesta 143, 10000, Zagreb, Croatia

Abstract

ABSTRACT Multiple-locus variable-number tandem-repeat analysis (MLVA), multiplex PCR, and PCR-restriction fragment length polymorphism analysis were compared for typing Brucella suis isolates. A perfect concordance was obtained among these molecular assays. However, MLVA was the only method to demonstrate brucellosis outbreaks and to confirm that wildlife is a reservoir for zoonotic brucellosis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01096-07

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