Isolation of Bacteroides fragilis Mutants with In Vivo Growth Defects by Using Tn 4400 ′, a Modified Tn 4400 Transposition System, and a New Screening Method-Reference-Cited by-同舟云学术

Isolation of Bacteroides fragilis Mutants with In Vivo Growth Defects by Using Tn 4400 ′, a Modified Tn 4400 Transposition System, and a New Screening Method

Published:2000-01 Issue:1 Volume:68 Page:415-419
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Tang Yixin P.¹,Malamy Michael H.¹

Affiliation:

1. Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111

Abstract

ABSTRACT A modified version of the Bacteroides fragilis transposon Tn 4400 , designated Tn 4400 ′, enabling rapid isolation and analysis of B. fragilis mutants has been constructed. To identify potential virulence factors, Tn 4400 ′-generated mutants were screened by a new method; this resulted in the isolation of 21 mutant strains with impaired growth characteristics on tissue culture monolayers but normal growth in rich medium anaerobically.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.68.1.415-419.2000

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