Antenatal Screening for Hepatitis B and Antibodies to Toxoplasma gondii and Rubella Virus: Evaluation of Two Commercial Immunoassay Systems-Reference-Cited by-同舟云学术

Antenatal Screening for Hepatitis B and Antibodies to Toxoplasma gondii and Rubella Virus: Evaluation of Two Commercial Immunoassay Systems

Published:2001-07 Issue:4 Volume:8 Page:785-787
ISSN:1071-412X
Container-title:Clinical Diagnostic Laboratory Immunology
language:en
Short-container-title:Clin Diagn Lab Immunol

Author:

Diepersloot Robert J. A.¹,Dunnewold-Hoekstra Hiske¹,Kruit-den Hollander Jorien¹,Vlaspolder Fer²

Affiliation:

1. Laboratory for Medical Microbiology, Diakonessen Hospital, Utrecht,1 and

2. Laboratory for Medical Microbiology, Alkmaar Medical Centre, Alkmaar,2 The Netherlands

Abstract

ABSTRACT A comparative evaluation of the Abbott AxSYM and DPC Immulite random-access analyzers was performed using 497 prospectively collected serum samples. These samples were sent to the laboratory for routine antenatal screening for hepatitis B surface antigen and immunoglobulin G (IgG) and IgM antibodies to Toxoplasma gondii and rubella virus. The overall agreement between the two assay systems ranged from 97.4 to 100%. After discrepancy analysis, the outcome in terms of sensitivity and specificity varied from 98.2 to 100% for all but one of the assays tested. The AxSYM rubella virus IgG assay tended to report protective or indeterminate antibody levels in 1% of the samples. This shortcoming might be overcome by raising the cutoff of the microparticle enzyme immunoassay system.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CDLI.8.4.785-787.2001

Reference14 articles.

1. Enders G. Infektionen und Impfungen in der Schwangerschaft. 1994 Urban & Schwarzenberg Munich Germany

2. The Abbott IMx automated benchtop immunochemistry analyzer system.;Fiore M.;Clin. Chem.,1988

3. Studies of the natural history and prevention of rubella.;Green R. H.;Am. J. Dis. Child.,1965

4. Fetal toxoplasmosis: outcome of pregnancy and infant follow-up after in utero treatment.;Hohlfeld P.;J. Pediatr.,1989

5. The significance of specific IgM antibody in the diagnosis of rubella antibody employing the immuno-fluorescent technique.;Iwakata S.;J. Can. Med. Assoc.,1972

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