Production of Bartonella Genus-Specific Monoclonal Antibodies-Reference-Cited by-同舟云学术

Production of Bartonella Genus-Specific Monoclonal Antibodies

Published:2001-07 Issue:4 Volume:8 Page:847-849
ISSN:1071-412X
Container-title:Clinical Diagnostic Laboratory Immunology
language:en
Short-container-title:Clin Diagn Lab Immunol

Author:

Liang Zhongxing¹,La Scola Bernard¹,Lepidi Hubert¹,Raoult Didier¹

Affiliation:

1. Unité des Rickettsies, CNRS UPRES-A 6020, Faculté de Médecine, Université de la Mediterranée, 13385 Marseille Cédex, France

Abstract

ABSTRACT Monoclonal antibodies (MAbs) which react with heat-resistant proteins with molecular masses of 32 to 33 kDa of 14 different Bartonella species were produced. These antibodies did not react with antigens of 26 diverse bacterial strains by microimmunofluorescence assay except MAb B3D4, which reacted with Chlamydia psittaci and Chlamydia trachomatis at low titers. The identification of a common Bartonella antigenic protein will make it possible to later produce a diagnostic antigen by cloning and expressing it in Escherichia coli . Moreover, these MAbs allow all Bartonella species to be identified to the genus level.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CDLI.8.4.847-849.2001

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