Affiliation:
1. Department of Biogeochemistry, Max-Planck-Institute for Terrestrial Microbiology, Marburg, Germany
Abstract
ABSTRACT
Homoacetogenic bacteria are versatile microbes that use the acetyl coenzyme A (acetyl-CoA) pathway to synthesize acetate from CO
2
and hydrogen. Likewise, the acetyl-CoA pathway may be used to incorporate other 1-carbon substrates (e.g., methanol or formate) into acetate or to homoferment monosaccharides completely to acetate. In this study, we analyzed the fractionation of pure acetogenic cultures grown on different carbon substrates. While the fractionation of
Sporomusa sphaeroides
grown on C
1
compounds was strong (ε
C1
, −49‰ to −64‰), the fractionation of
Moorella thermoacetica
and
Thermoanaerobacter kivui
using glucose (ε
Glu
= −14.1‰) was roughly one-third as strong, suggesting a contribution of less-depleted acetate from fermentative processes. For
M. thermoacetica
, this could indeed be validated by the addition of nitrate, which inhibited the acetyl-CoA pathway, resulting in fractionation during fermentation (ε
ferm
= −0.4‰). In addition, we determined the fractionation into microbial biomass of
T. kivui
grown on H
2
/CO
2
(ε
anabol.
= −28.6‰) as well as on glucose (ε
anabol.
= +2.9‰).
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
22 articles.
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