Cloning, sequence, and properties of the soluble pyridine nucleotide transhydrogenase of Pseudomonas fluorescens

Author:

French C E1,Boonstra B1,Bufton K A1,Bruce N C1

Affiliation:

1. Institute of Biotechnology, University of Cambridge, United Kingdom.

Abstract

The gene encoding the soluble pyridine nucleotide transhydrogenase (STH) of Pseudomonas fluorescens was cloned and expressed in Escherichia coli. STH is related to the flavoprotein disulfide oxidoreductases but lacks one of the conserved redox-active cysteine residues. The gene is highly similar to an E. coli gene of unknown function.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference21 articles.

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3. Fisher R. R. and S. R. Earle. 1982. Membrane-bound pyridine nucleotide transhydrogenases p. 279-324. In J. Everse B. Anderson and K.-S. You (ed.) The pyridine nucleotide coenzymes. Academic Press Inc. New York N.Y.

4. Genetics Computer Group. 1994. Program manual for the Wisconsin package version 8 September 1994. Genetics Computer Group Madison Wis.

5. Glutathione reductase from Escherichia coli: cloning and sequence analysis of the gene and relationship to other flavoprotein disulphide oxidoreductases;Greer S.;Biochemistry,1986

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