Affiliation:
1. Departments
of Medicine, Pathology, and Molecular Microbiology, Washington
University School of Medicine, St. Louis, Missouri
63110
Abstract
ABSTRACT
Alpha-2a
interferon (IFN-α2a) has beneficial clinical effects on human
T-cell leukemia virus type 1 (HTLV-1) infection, but its antiviral
mechanism of action is unknown. Antiviral effects of IFN-α2a
were studied in 293T cells expressing HTLV-1 proviral DNA and in
HTLV-1-infected cells (HOS/PL, MT2, and HUT102). In 293T cells, an
50% inhibitory concentration of 10 U of
IFN-α2a/ml was determined by p19 antigen ELISA. Analysis of
IFN-treated cells demonstrated no defect in viral protein synthesis but
did show a decrease in the level of released virus, as determined by
immunoblot assays. Electron microscopy studies of IFN-treated cells
revealed neither a defect in the site of virus budding nor tethering of
virus particles at the plasma membrane, thus arguing against an effect
on virus release. Cell fractionation studies and confocal microscopy
showed no effect of IFN on Gag association with membranes. However, the
level of Gag association with lipid rafts was decreased, suggesting a
role of IFN in inhibiting HTLV-1
assembly.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Cited by
49 articles.
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