Characterization of an Immunogenic Glycocalyx on the Surfaces of Cryptosporidium parvum Oocysts and Sporozoites

Author:

Nanduri Jayasri1,Williams Selvi2,Aji Toshiki3,Flanigan Timothy P.4

Affiliation:

1. Institute of Pathology, Case Western Reserve University, Cleveland, Ohio1;

2. Brown University School of Medicine,2 Providence, Rhode Island; and

3. Department of Parasitology, Okayama University, Okayama, Japan3

4. The Miriam Hospital,4

Abstract

ABSTRACT Ruthenium red staining of Cryptosporidium parvum oocysts revealed the presence of a carbohydrate matrix on their outer bilayers that is characteristic of a glycocalyx. Surface labeling of intact oocysts identified material of high molecular weight (>10 6 ) that reacted positively with sera from cryptosporidium-infected patients and with immunoglobulin A monoclonal antibodies.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference16 articles.

1. Ultrastructure, carbohydrate, and amino acid analysis of two preparations of the cercarial glycocalyx of Schistosoma mansoni;Caulfield J. P.;J. Parasitol.,1987

2. Asexual development of Cryptosporidium parvum within a differentiated human enterocyte cell line

3. Cryptosporidiosis;Flanigan T. P.;Prog. Clin. Parasitol.,1993

4. Adherence of Salmonella typhimurium to Caco-2 cells: identification of a glycoconjugate receptor

5. Schemata for the production of monoclonal antibody-producing hybridomas;Kipps T. J.;Handb. Exp. Immunol.,1986

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