Species-Level Identification of Orthopoxviruses with an Oligonucleotide Microchip-Reference-Cited by-同舟云学术

Species-Level Identification of Orthopoxviruses with an Oligonucleotide Microchip

Published:2002-03 Issue:3 Volume:40 Page:753-757
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Lapa Sergey¹,Mikheev Maxim²,Shchelkunov Sergei²,Mikhailovich Vladimir¹,Sobolev Alexander¹,Blinov Vladimir²,Babkin Igor²,Guskov Alexander²,Sokunova Elena²,Zasedatelev Alexander¹,Sandakhchiev Lev²,Mirzabekov Andrei¹

Affiliation:

1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow

2. State Research Center of Virology and Biotechnology “Vector,” Koltsovo, Novosibirsk Region, Russia

Abstract

ABSTRACT A method for species-specific detection of orthopoxviruses pathogenic for humans and animals is described. The method is based on hybridization of a fluorescently labeled amplified DNA specimen with the oligonucleotide DNA probes immobilized on a microchip (MAGIChip). The probes identify species-specific sites within the crmB gene encoding the viral analogue of tumor necrosis factor receptor, one of the most important determinants of pathogenicity in this genus of viruses. The diagnostic procedure takes 6 h and does not require any sophisticated equipment (a portable fluorescence reader can be used).

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.3.753-757.2002

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