Method for Rapid Detection of Cyanogenic Bacteria

Author:

Castric Kathleen F.1,Castric Peter A.1

Affiliation:

1. Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania 15282

Abstract

An agar plate method is described in which the production of hydrogen cyanide by as many as 50 microbial isolates per plate may be detected. Cyanide produced by the organisms reacts with copper(II) ethylacetoacetate and 4,4′-methylenebis-( N,N -dimethylaniline) in a paper disk suspended above the microbial colonies. Cell growth occurs in depressions in the agar surface, which allows separation of colonies and enhances sensitivity of hydrogen cyanide detection.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference10 articles.

1. Bunch A. W. and C. J. Knowles. 1981. The effect of growth conditions on cyanogenesis by the Snow Mould fungus p. 311-319. In B. Vennesland E. E. Conn C. J. Knowles J. Westley and F. Wissing (ed.) Cyanide in biology. Academic Press London.

2. Hydrogen cyanide, a secondary metabolite of Pseudomonas aeruginosa;Castric P. A.;Can. J. Microbiol.,1975

3. Castric P. A. 1981. The metabolism of hydrogen cyanide by bacteria p. 233-261. In B. Vennesland E. E. Conn C. J. Knowles J. Westley and F. Wissing (ed.) Cyanide in biology. Academic Press London.

4. Replacement of benzidine by copper ethylacetoacetate and tetrabase as spot-test reagent for hydrogen cyanide and cyanogen;Feigl F.;Analyst (London),1966

5. Krutz H. 1981. Different origins of cyanide contamination in small rivers p. 479-485. In B. Vennesland E. E. Conn C. J. Knowles J. Westley and F. Wissing (ed.) Cyanide in biology. Academic Press London.

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