Reduction and Reoxidation of Australia Antigen: Loss and Reconstitution of Particle Structure and Antigenicity

Author:

Sukeno Noriyoshi1,Shirachi Ryoichi1,Yamaguchi Junji1,Ishida Nakao1

Affiliation:

1. Department of Bacteriology, School of Medicine, and The Research Institute for Tuberculosis, Leprosy, and Cancer, Tohoku University, Sendai, Japan

Abstract

Reduction of Australia antigen with dithiothreitol resulted in the loss of antigenicity and morphological integrity. Oxidation of the reduced Australia antigen reconstituted both of these characters.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference8 articles.

1. Some antigenic and physical properties of virus-like particles in sera of hepatitis patients;Barker L. F.;J. Immunol.,1969

2. Dithiothreitol, a new protective reagent for SH groups;Clealand W. W.;Biochemistry,1964

3. Australia antigen: large-scale purification from human serum and biochemical studies of its proteins;Gerin J. L.;J. Virol.,1971

4. Biophysical properties of Auistralia antigen;Gerin J. L.;J. Virol.,1969

5. Rapid detection of Australia antigen by counterimmunoelectrophoresis;Gocke D. J.;J. Immunol.,1970

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