Equilibrium Constant for Conversion of Pyruvate to Acetyl Phosphate and Formate-Reference-Cited by-同舟云学术

Equilibrium Constant for Conversion of Pyruvate to Acetyl Phosphate and Formate

Published:1971-12 Issue:3 Volume:108 Page:1107-1111
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Tanaka Nobumasa¹,Johnson Marvin J.¹

Affiliation:

1. The Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706

Abstract

Preparations of pyruvate formate-lyase were made from Escherichia coli cells. Net reversal of the “phosphoroclastic split” of pyruvate was readily demonstrated with these preparations. Incubation of acetyl phosphate with formate resulted in the accumulation of pyruvate in concentrations up to 0.5 m m . Catalytic amounts of coenzyme A were essential. Pyruvate was also readily formed from acetyl coenzyme A and formate. The equilibrium constant of the reaction (pyruvate − + HPO 4 2− → acetyl phosphate 2− + formate − ) has been determined to be about 23 at 37 C.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.108.3.1107-1111.1971

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