Affiliation:
1. Unité de Programmation Moléculaire et Toxicologie Génétique, Institut Pasteur, 75724 Paris cedex 15, France,1 and
2. Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada2
Abstract
ABSTRACT
The 72
Escherichia coli
strains of the ECOR collection were examined for resistance to 10 different antimicrobial agents including ampicillin, tetracycline, mercury, trimethoprim, and sulfonamides. Eighteen strains were resistant to at least one of the antibiotics tested, and nearly 20% (14 of 72) were resistant to two or more. Several of the resistance determinants were shown to be carried on conjugative elements. The collection was screened for the presence of the three classes of integrons and for the
sul1
gene, which is generally associated with class 1 integrons. The four strains found to carry a class 1 integron also had Tn
21
-encoded mercury resistance. One of the integrons encoded a novel streptomycin resistance gene,
aadA7
, with an
attC
site (or 59-base element) nearly identical to the
attC
site associated with the
qacF
gene cassette found in In40 (M.-C. Ploy, P. Courvalin, and T. Lambert, Antimicrob. Agents Chemother. 42:2557–2563, 1998). The conservation of associated
attC
sites among unrelated resistance cassettes is similar to arrangements found in the
Vibrio cholerae
superintegrons (D. Mazel, B. Dychinco, V. A. Webb, and J. Davies, Science 280:605–608, 1998) and supports the hypothesis that resistance cassettes are picked up from superintegron pools and independently assembled from unrelated genes and related
attC
sites.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Cited by
265 articles.
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