Affiliation:
1. Institute of Molecular Genetics and Genetic Engineering, 11001 Belgrade, Yugoslavia
Abstract
ABSTRACT
In previous studies we demonstrated that mutations in the genes
cysB
,
cysE
, and
cls
(
nov
) affect resistance of
Escherichia coli
to novobiocin (J. Rakonjac, M. Milic, and D. J. Savic, Mol. Gen. Genet. 228:307–311, 1991; R. Ivanisevic, M. Milic, D. Ajdic, J. Rakonjac, and D. J. Savic, J. Bacteriol. 177:1766–1771, 1995). In this work we expand this list with mutations in
rpoN
(the gene for RNA polymerase subunit ς
54
) and the tRNA synthetase genes
alaS
,
argS
,
ileS
, and
leuS
. Similarly to resistance to the penicillin antibiotic mecillinam, resistance to novobiocin of tRNA synthetase mutants appears to depend upon the RelA-mediated stringent response. However, at this point the overlapping pathways of mecillinam and novobiocin resistance diverge. Under conditions of stringent response induction, either by the presence of tRNA synthetase mutations or by constitutive production of RelA protein, inactivation of the
cls
gene diminishes resistance to novobiocin but not to mecillinam.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference25 articles.
1. Segregation of new lysogenic types during growth of a double lysogenic strain derived from Escherichia coli K12;Appleyard R. K.;Genetics,1954
2. XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection;Bullock W. O.;BioTechniques,1987
3. The stringent response;Cashel M.;Escherichia coli and Salmonella: cellular and molecular biology,1996
4. gyrB mutations which confer coumarin resistance also affect DNA supercoiling and ATP hydrolysis by Escherichia coli DNA gyrase;Contreras A.;Mol. Microbiol.,1992
5. Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase;Gellert M.;Proc. Natl. Acad. Sci. USA,1976
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