Abstract
Virus-like particles were purified from stools of patients in an epidemic of hepatitis A in Germany. When reference MS-1 chimpanzee pre-inoculation and convalescent sera were used, the close serological relationship of the purified particles to well-known isolates of hepatitis A could be established. On the other hand, the physicochemical characteristics of the particles were determined in parallel to the characteristics of a marker parvovirus (LuIII) and a marker picornavirus (poliovirus type 2). It could be shown that the majority of the hepatitis A-associated particles band at 1.34 g/ml in CsCl and, like poliovirus, sediment at about 160S. In addition, a distinct hepatitis A antigen was observed, which banded at 1.305 g/ml and sedimented between 50 and 90S. A further component accumulated in the density range of between 1.38 and 1.44 g/ml. However, it seemed to be rather labile. Upon reisolation from CsCl and sedimentation in sucrose, it resolved into a 160S, a 90 to 100S, and a 50S form. The size of the 160S particles (27 to 29 nm) could be readily distinguished from that of the parvovirus (22 to 24 nm). It is concluded, therefore, that hepatitis A-associated virus particles are more likely to be classified with the picornaviruses than with the parvoviruses.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference30 articles.
1. Studies on an attenuated poliomyelitis virus type 2, purification and physicochemical characteristics of the virus;Anderer F. A.;Z. Naturforsch. Teil B,1964
2. Isolation and characterization of hepatitis A virus;Bradley D. W.;Am. J. Clin. Pathol.,1976
3. Purification of hepatitis A virus from chimpanzee stools;Bradley D. W.;J. Virol.,1977
4. CsCl banding of hepatitis A-associated virus-like particles;Bradley D. W.;J. Infect. Dis.,1975
5. Virus assembly. Annu;Casjens S.;Rev. Biochem.,1975
Cited by
65 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
1. The yielding behaviour of human mucus;Advances in Colloid and Interface Science;2023-12
2. Bioaerosol Dynamics;Bioaerosol Characterisation, Transportation and Transmission;2023
3. The History of Hepatitis A;Clinical Liver Disease;2020-10
4. Energy Requirements for Loss of Viral Infectivity;Food and Environmental Virology;2020-08-05
5. The hepatitis A virus infection;Liver;2008-12-10