Author:
Chandler M S,Morrison D A
Abstract
To identify and map genes involved in competence for genetic transformation in Streptococcus pneumoniae, we have cloned DNA surrounding an ermB insertion mutation that causes a competence factor deficiency. We recovered the insert and approximately 500 base pairs of neighboring pneumococcal DNA in pMB9. Larger pieces of DNA from this region were unstable in pMB9 and pBR325. However, larger pieces were stable in pKK232-8, an Escherichia coli vector containing strong transcription terminators. Overlapping pieces of wild-type DNA from this competence control region were cloned and mapped in this vector. Insertion mutations were constructed in vitro throughout the cloned region. When crossed into the pneumococcus chromosome, they showed that the com locus was 4.2 to 5.2 kilobases long.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference49 articles.
1. A rapid alkaline extraction procedure for screening recombinant plasmid DNA;Birnboim H. C.;Nucleic Acids Res.,1979
2. Plasmid vectors for the selection of promoters;Brosius J.;Gene,1984
3. Identification of tetracycline-resistant Rplasmids in Streptococcus agalactiae (group B);Burdett V.;Antimicrob. Agents Chemother.,1980
4. Chandler M. S. and D. A. Morrison. 1987. Molecular cloning of a competence control region from Streptococcus pneumoniae by use of transcription terminator vectors in Escherichia coli p. 193-196. In J. J. Ferretti and R. Curtiss III (ed.) Streptococcal genetics. Amenican Society for Microbiology Washington D.C.
5. Identification of Streptococcus pneumoniae mismatch repair genes by an additive transformation approach;Claverys J. P.;Mol. Gen. Genet.,1984
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