Transformation of the archaebacterium Halobacterium volcanii with genomic DNA-Reference-Cited by-同舟云学术

Transformation of the archaebacterium Halobacterium volcanii with genomic DNA

Published:1989-09 Issue:9 Volume:171 Page:4987-4991
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Cline S W¹,Schalkwyk L C¹,Doolittle W F¹

Affiliation:

1. Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada.

Abstract

We describe optimization of a transformation system for the halophilic archaebacterium Halobacterium volcanii. Transformation of spheroplasts in the presence of polyethylene glycol permits the uptake and expression of high-molecular-weight linear fragments of genomic DNA as well as plasmid or bacteriophage DNA. Transformations can be performed with either fresh or frozen cell preparations. Auxotrophic mutants were transformed to prototrophy with genomic DNA from wild-type cells with efficiencies of 5 x 10(4)/micrograms of DNA and frequencies of 8 x 10(-5) per regenerated spheroplast. The overall efficiency of transformation with genomic DNA implies that genetic recombination is an efficient process in H. volcanii.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.171.9.4987-4991.1989

Reference21 articles.

1. Transformation and DNA size. I. Activity of fragments of defined size and a fit to a random double cross-over model;Cat Q;J. Mol. Biol.,1968

2. Genome mapping in halobacteria;Charlebois R. L.;Can. J. Microbiol.,1989

3. Characterization of pHV2 from Halobacterium volcanii and its use in demonstrating transformation of an archaebacterium;Charlebois R. L.;Proc. Natl. Acad. Sci. USA,1987

4. Separation of large DNA molecules by contour-clamped homogeneous electric fields;Chu G.;Science,1986

5. Efficient transfection of the archaebacterium Halobacterium halobium;Cline S. W.;J. Bacteriol.,1987

Cited by 124 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Haloarchaea as Promising Chassis to Green Chemistry;Microorganisms;2024-08-22

2. A vector system for single and tandem expression of cloned genes and multi-colour fluorescent tagging in Haloferax volcanii;Microbiology;2024-05-24

3. Dynamic self-association of archaeal tubulin-like protein CetZ1 drivesHaloferax volcaniimorphogenesis;2024-04-08

4. A vector system for single and tandem expression of cloned genes and multi-colour fluorescent tagging inHaloferax volcanii;2024-03-13

5. Extracellular vesicle formation in Euryarchaeota is driven by a small GTPase;Proceedings of the National Academy of Sciences;2024-02-26