Affiliation:
1. Department of Biological Sciences, University of Calgary, Alberta, Canada.
Abstract
The nucleotide sequence of a 2.0-kilobase-pair EcoRI restriction fragment upstream from the gene (rub, 162 base pairs) encoding rubredoxin from Desulfovibrio vulgaris Hildenborough indicates that it is part of a larger transcriptional unit, containing an additional 378-base-pair open reading frame which terminates 16 nucleotides from the translational start of the rub gene and could encode a polypeptide of 14 kilodaltons (kDa). Northern (RNA) blotting of RNA isolated from both D. vulgaris Hildenborough and Escherichia coli TG2 transformed with plasmid pJK29, which contains both genes on a 1.1-kilobase-pair SalI insert, confirms that the genes for this 14-kDa polypeptide and rubredoxin are present on a single transcript of 680 nucleotides. Strong evidence that the 14-kDa polypeptide is also a redox protein is provided by the fact that its NH2 terminus is homologous to desulforedoxin, which has been isolated from D. gigas as a small dimeric redox protein (36 amino acids per monomer), coordinating two iron atoms. Since rubredoxin is a potential redox partner for the 14-kDa protein, it has been tentatively named rubredoxin oxidoreductase, produced by the rbo gene. Southern blotting indicates that the rbo-rub operon is present in several species and strains of sulfate-reducing bacteria.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference44 articles.
1. The structure of a bacterial ferredoxin;Adman E. T.;J. Biol. Chem.,1973
2. A structural model of rubredoxin from Desulfovibrio vulgaris at 2 A resolution;Adman E. T.;J. Mol. Biol.,1977
3. Non-heme iron proteins. IV. Structural studies of Micrococcus aerogenes (Peptococcus aerogenes) rubredoxin;Bachmayer H.;Biochemistry,1968
4. Non-heme iron proteins. V. The amino acid sequence of rubredoxin from Peptostreptococcus elsdenii;Bachmayer H.;J. Biol. Chem.,1968
5. Bankier A. T. and B. G. Barrell. 1983. Shotgun DNA sequencing p. 1-34. In R. A. Flavell (ed.) Techniques in the life sciences B5. Nucleic acid biochemistry. Elsevier Scientific Publishers Ireland Ltd. Shannon Ireland.
Cited by
84 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献