BIOCHEMICAL STUDIES ON STAPHYLOCOAGULASE AND AN ALLIED PHOSPHATASE ACTIVITY

Author:

Inniss William E.1,Sanclemente Charles L.1

Affiliation:

1. Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan

Abstract

Inniss, William E. (Michigan State University, East Lansing) and Charles L. SanClemente . Biochemical studies on staphylocoagulase and an allied phosphatase activity. J. Bacteriol. 83: 941–947. 1962.—The present investigation was undertaken to determine whether the reported correlation between the coagulase and phosphatase activity of the staphylococci was functional. Staphylococcus aureus , phage-propagating strain 70, grown in brain heart infusion was the source of the purified coagulase. The concomitant phosphatase activity, measured spectrophotometrically at 400 mμ using p -nitrophenylphosphate as substrate, showed a parallel decrease during thermal inactivation at 37 and 56 C. Anion-exchange chromatography and electrophoresis using starch, starch gel, and paper as stabilizing media failed to separate the two activities. Since iodoacetate, ethylenediamine-tetraacetate, fluoride, azide, and p -chloromercuribenzoate always exerted different degrees of inactivation, apparently the same mechanism was not involved. This supposition was supported by subsequent saturation of the phosphatase with excess substrate (100-fold K s value) and the demonstration that under this condition coagulase was not inhibited. During this purification process, comparable increases in specific activity occurred for both coagulase and phosphatase, indicating the presence of a common protein carrier.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference20 articles.

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2. Staphylococcal phosphatase, glucuronidase and sulphatase;BARBER M., B.;J. Pathol. Bacteriol.,1951

3. Identification of Staphylococcus pyogenes by the phosphatase reaction;BARBER M.;J. Pathol. Bacteriol.,1951

4. A quantitative study of the phosphatase activity of Micrococcus pyogenes;BARNES E. H.;J. Bacteriol.,1957

5. The bacteriophage typing of staphylococci;BLAIR J. E.;J. Infectious Diseases,1953

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1. INVESTIGATIONS ON THE ENZYMES AND TOXINS OF STAPHYLOCOCCI;Acta Pathologica Microbiologica Scandinavica;2009-08-18

2. INVESTIGATIONS ON THE ENZYMES AND TOXINS OF STAPHYLOCOCCI;Acta Pathologica Microbiologica Scandinavica;2009-08-17

3. INVESTIGATIONS ON THE ENZYMES AND TOXINS OF STAPHYLOCOCCI;Acta Pathologica Microbiologica Scandinavica;2009-08-17

4. STAPHYLOCOAGULASE *;Annals of the New York Academy of Sciences;2006-12-16

5. PURIFICATION AND CHARACTERIZATION OF STAPHYLOCOAGULASE;Journal of Bacteriology;1963-09

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