Nucleotide sequence of SHV-2 beta-lactamase gene

Author:

Garbarg-Chenon A1,Godard V1,Labia R1,Nicolas J C1

Affiliation:

1. Laboratoire de Microbiologie, Hopital Trousseau, Paris, France.

Abstract

The nucleotide sequence of plasmid-mediated beta-lactamase SHV-2 from Salmonella typhimurium (SHV-2pHT1) was determined. The gene was very similar to chromosomally encoded beta-lactamase LEN-1 of Klebsiella pneumoniae. Compared with the sequence of the Escherichia coli SHV-2 enzyme (SHV-2E.coli) obtained by protein sequencing, the deduced amino acid sequence of SHV-2pHT1 differed by three amino acid substitutions.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference14 articles.

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2. Close evolutionary relationship between the chromosomally encoded p-lactamase gene of KlebsieWa pneumoniae and the TEM 5-lactamase gene mediated by R plasmids;Arakawa Y.;FEDS Lett.,1986

3. Single amino acid substitution between SHV-1 -lactamase and cefotaxime-hydrolyzing SHV-2 enzyme;Barth M.;FEBS Lett.,1988

4. Complete amino acid sequence of p453-plasmid-mediated PIT-2 1-lactamase (SHV-1);Barthelemy M.;Biochem. J.,1988

5. Dissemination in five French hospitals of Klebsiella pneumoniae serotype K25 harboring a new transferable enzymatic resistance to third-generation cephalosporins and aztreonam;Bur E;Eur. J. Clin. Microbiol.,1988

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