Author:
Hunt D E,Jones J V,Dowell V R
Abstract
Bacteroides gingivalis has been implicated in various forms of periodontal disease and may be responsible for other diseases in humans. The role of B. gingivalis in disease has been difficult to assess, because it is inhibited by most selective media commonly used by clinical laboratories to aid in isolating gram-negative, nonsporeforming anaerobes. We have developed a new medium, Bacteroides gingivalis agar, which contains bacitracin, colistin, and nalidixic acid as selective agents. This medium allowed B. gingivalis to be isolated from oral specimens with little difficulty and also allowed B. gingivalis to be isolated from phenotypically similar Bacteroides species, such as B. asaccharolyticus and B. endodontalis, with which it can easily be confused.
Publisher
American Society for Microbiology
Reference23 articles.
1. Allen S. D. J. A. Siders and L. M. Marler. 1985. Isolation and examination of anaerobic bacteria p. 413-433. In E. H. Lennette A. Balows W. J. Hausler Jr. and H. J. Shadomy (ed.) Manual of clinical microbiology 4th ed. American Society for Microbiology Washington D.C.
2. Genetic heterogeneity in Bac-teroides asaccharolyticus (Holdeman and Moore 1970) Finegold and Barnes 1977 (approved Lists, 1980) and proposal of Bacteroides gingivalis sp. nov. and Bacteroides macacae (Slots and Genco) comb. nov;Coykendall A. L.;Int. J. Syst. Bacteriol.,1980
3. Dowell V. R. Jr. G. L. Lombard F. S. Thompson and A. Y. Armfield. 1977. Media for isolation characterization and identification of obligately anaerobic bacteria p. 4. Centers for Disease Control Atlanta Ga.
4. A new culture medium for medical bacteriology;ElIner P. D.;Am. J. Clin. Pathol.,1966
5. Report on the ICSB taxonomic subcommittee on gram-negative anaerobic rods. Proposal that the saccharolytic and asaccharolytic strains at present classified in the species Bacteroides melaninogenicus (Oliver and Wherry) be reclassified in two species as Bacteroides melaninogenicus and Bacteroides asaccharolyticus;Finegold S. M.;Int. J. Syst. Bacteriol.,1977