Affiliation:
1. University of Wisconsin-Parkside, Kenosha, Wisconsin 53140
Abstract
Myxamoebae of the acellular slime mold
Physarum polycephalum
have been cultured axenically in a soluble medium. The growth medium contains bovine serum albumin, embryo extracts, liver infusion broth, peptone, and glucose. Cell densities ranging from 3 × 10
6
to 5 × 10
6
cells/ml have been obtained with this medium. To date, myxamoebae have been serially transferred more than 100 times without deleterious effect.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
40 articles.
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