Characterization of Hemolysin of Moraxella bovis Using a Hemolysis-Neutralizing Monoclonal Antibody

Author:

Billson F. Mark1,Harbour Colin2,Michalski Wojtek P.3,Tennent Jan M.3,Egerton John R.1,Hodgson Jennifer L.1

Affiliation:

1. Department of Veterinary Clinical Sciences, University of Sydney, Camden, New South Wales 2570,1

2. Department of Infectious Diseases, University of Sydney, Sydney 2000,2and

3. CSIRO, Division of Animal Health, Australian Animal Health Laboratory, Geelong, Victoria 3220,3Australia

Abstract

ABSTRACT A concentrated bacterial culture supernatant from the hemolytic Moraxella bovis strain UQV 148NF was used to immunize mice and generate monoclonal antibodies (MAbs). One, MAb G3/D7, neutralized the hemolytic activity of M. bovis and recognized a 94-kDa protein by Western blot analysis in hemolytic M. bovis strains representing each of the different fimbrial serogroups. Exposure of corneal epithelial cells to M. bovis concentrated culture supernatants demonstrated a role for an exotoxin in the pathogenesis of infectious bovine keratoconjunctivitis, while neutralization of hemolytic and cytotoxic activities by MAb G3/D7 implies that these activities are related or have common epitopes. The action of M. bovis hemolysin was further characterized in sheep erythrocyte preparations with a binding step and Ca 2+ required for lysis to proceed, similar to the RTX family of bacterial exotoxins. Neutralization of lytic activity in vitro is evidence for the presence of M. bovis antigens, which may be capable of protecting cattle from the development of infectious bovine keratoconjunctivitis.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference39 articles.

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