Isolation of temperature-sensitive mutants of R plasmid by in vitro mutagenesis with hydroxylamine-Reference-Cited by-同舟云学术

Isolation of temperature-sensitive mutants of R plasmid by in vitro mutagenesis with hydroxylamine

Published:1976-09 Issue:3 Volume:127 Page:1561-1563
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Hashimoto T,Sekiguchi M

Abstract

Temperature-sensitive mutants were isolated from a tetracycline resistance plasmid, pSC101, after mutagenesis with hydroxylamine. They were divided into three classes according to their growth characteristics. They include mutants defective in the replication of plasmid deoxyribonucleic acid and strains having a mutation in the drug resistance gene.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.127.3.1561-1563.1976

Reference5 articles.

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3. Transformation of Salmonella typhymurium by plasmid deoxyribonucleic acid;Lederberg E. M.;J. Bacteriol.,1974

4. Isolation and characterization of a temperature-sensitive amber suppressor mutant of Escherichia coli K 12;Nagata T.;Mol. Gen. Genet.,1973

5. Mutagenic treatment of double- and single-stranded DNA phages T4 and S13 with hydroxylamine;Tessman I.;Virology,1968

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