Evaluation of Epstein-Barr Virus, Human Herpesvirus 6 (HHV-6), and HHV-8 Antiviral Drug Susceptibilities by Use of Real-Time-PCR-Based Assays

Author:

Bounaadja Lotfi1,Piret Jocelyne1,Goyette Nathalie1,Boivin Guy1

Affiliation:

1. Infectious Disease Research Centre, Laval University, Quebec City, Quebec, Canada

Abstract

ABSTRACT Evaluation of candidate antiviral drugs against Epstein-Barr virus (EBV), human herpesvirus 6 (HHV-6), and HHV-8 is hampered by the lack of convenient laboratory assays. We developed real-time quantitative PCR assays performed on supernatants of lymphoma cell lines and determined the 50% inhibitory concentrations (IC 50 s) of nucleoside, nucleotide, and pyrophosphate analogues against these herpesviruses.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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