Optimization of Procedures for Isolation of Mycobacteria from Soil and Water Samples Obtained in Northern India-Reference-Cited by-同舟云学术

Optimization of Procedures for Isolation of Mycobacteria from Soil and Water Samples Obtained in Northern India

Published:2004-06 Issue:6 Volume:70 Page:3751-3753
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Parashar Deepti¹,Chauhan D. S.¹,Sharma V. D.¹,Chauhan Aradhana¹,Chauhan S. V. S.²,Katoch V. M.¹

Affiliation:

1. Central JALMA Institute for Leprosy and Other Mycobacterial Diseases (Indian Council of Medical Research)

2. School of Life Sciences, Dr. B. R. Ambedkar University, Agra, India

Abstract

ABSTRACT For isolation of environmental mycobacteria, a decontamination procedure has been standardized by which treatment with 3% sodium dodecyl sulfate plus 4% NaOH (15 and 30 min for rapid and slow growers, respectively) is followed by incubation with 2% cetrimide (5 and 15 min for fast- and slow-growing mycobacteria, respectively); this procedure was found to completely eliminate contamination with other organisms and resulted in the isolation of only mycobacteria.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.70.6.3751-3753.2004

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