Comparison of ZMAC and MARC-145 Cell Lines for Improving Porcine Reproductive and Respiratory Syndrome Virus Isolation from Clinical Samples

Author:

Yim-im Wannarat1,Huang Haiyan1,Park Jie1,Wang Chong1,Calzada Gabriela2,Gauger Phillip1ORCID,Harmon Karen1,Main Rodger1,Zhang Jianqiang1ORCID

Affiliation:

1. Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa, USA

2. Aptimmune Biologics, St. Louis, Missouri, USA

Abstract

The MARC-145 cell line is commonly used to isolate porcine reproductive and respiratory syndrome virus (PRRSV) for diagnostics, research, and vaccine production, but it yields frustratingly low success rates of virus isolation (VI). The ZMAC cell line, derived from porcine alveolar macrophages, has become available, but its utilization for PRRSV VI from clinical samples has not been evaluated.

Funder

American Association of Swine Veterinarians Foundation

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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