Comparison of ZMAC and MARC-145 Cell Lines for Improving Porcine Reproductive and Respiratory Syndrome Virus Isolation from Clinical Samples-Reference-Cited by-同舟云学术

Comparison of ZMAC and MARC-145 Cell Lines for Improving Porcine Reproductive and Respiratory Syndrome Virus Isolation from Clinical Samples

Published:2021-02-18 Issue:3 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Yim-im Wannarat¹,Huang Haiyan¹,Park Jie¹,Wang Chong¹,Calzada Gabriela²,Gauger Phillip¹^ORCID,Harmon Karen¹,Main Rodger¹,Zhang Jianqiang¹^ORCID

Affiliation:

1. Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa, USA

2. Aptimmune Biologics, St. Louis, Missouri, USA

Abstract

The MARC-145 cell line is commonly used to isolate porcine reproductive and respiratory syndrome virus (PRRSV) for diagnostics, research, and vaccine production, but it yields frustratingly low success rates of virus isolation (VI). The ZMAC cell line, derived from porcine alveolar macrophages, has become available, but its utilization for PRRSV VI from clinical samples has not been evaluated.

Funder

American Association of Swine Veterinarians Foundation

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01757-20

Reference48 articles.

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