Affiliation:
1. Department of Agricultural Microbiology, Rutgers, The State University, New Brunswick, New Jersey
Abstract
Coscarelli, Waldimero
(Rutgers, The State University, New Brunswick, N.J.)
and David Pramer
. Nutrition and growth of
Arthrobotrys conoides
. J. Bacteriol.
84:
60–64. 1962—Despite its ability to capture, kill, and subsist on nematodes, the predacious fungus
Arthrobotrys conoides
does not demonstrate a unique nutritional pattern. Yeast extract, required for growth in a glucose-inorganic salts medium, was replaced by biotin, thiamine, and zinc. Biotin biosynthesis by the fungus is blocked at the desthiobiotin to biotin conversion.
A. conoides
is unable to synthesize either of the two moieties of thiamine but, when supplied with pyrimidine and thiazole, completes biosynthesis of the vitamin via a coupling reaction. A chemically defined medium that supports rapid and abundant growth of
A. conoides
is described. Glucose is utilized efficiently (economic coefficient of 31.8 to 42.8), and yields exceeding 9 g (dry wt) of fungus tissue per liter of medium are obtained.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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