Recovery of Dengue Viruses from Tissues of Experimentally Infected Rhesus Monkeys-Reference-Cited by-同舟云学术

Recovery of Dengue Viruses from Tissues of Experimentally Infected Rhesus Monkeys

Published:1972-09 Issue:3 Volume:24 Page:328-333
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Marchette Nyven J.¹²,Halstead Scott B.¹²,Nash Donald R.¹²,Stenhouse Andrew C.¹²

Affiliation:

1. Department of Tropical Medicine and Medical Microbiology, University of Hawaii School of Medicine, Honolulu, Hawaii 96816

2. Leahi Hospital, Honolulu, Hawaii 96816

Abstract

A tissue explant culture technique for the recovery of dengue virus from experimentally infected monkey tissue is described and compared with tissue culture assay of tissue triturates and co-cultivation of trypsinized cells in cell cultures. The most efficient technique was one in which minced tissue was explanted in co-culture with dengue virus-susceptible LLC-MK2 monkey kidney cells. This technique shows promise of being useful for detection of virus in autopsy material from fatal dengue hemorrhagic fever cases.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.24.3.328-333.1972

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