Diversity of Structures Carrying the High-Level Gentamicin Resistance Gene (aac6-aph2) in Enterococcus faecalis Strains Isolated in France

Author:

Casetta Anne1,Hoï Annie Buu2,de Cespédès Gilda1,Horaud Thea1

Affiliation:

1. Laboratoire des Staphylocoques et des Streptocoques, Institut Pasteur,1 and

2. Laboratoire de Microbiologie, Hôpital Broussais,2 Paris, France

Abstract

ABSTRACT Of 24 high-level gentamicin-resistant clinical isolates of Enterococcus faecalis , 20 carried gentamicin resistance (Gm r ) plasmids. The plasmids ranged from 65.0 to 80.0 kb in size. Three of these plasmids were nonconjugative, and 17 transferred by conjugation to an E. faecalis recipient at low frequency (10 −5 to 10 −6 transconjugants per donor). The remaining four strains had a nonconjugative chromosomal Gm r determinant. On the basis of restriction enzyme and DNA-DNA hybridization profiles, Tn 4001 -like α elements were located on the chromosome and three types of Tn 4001 -truncated structures, I, II, and III, were found to be carried by the Gm r plasmids. Structure I lacked IS 256 in the right-hand flanking extremity of Tn 4001 . Structure II was the same as structure I except that it also had a partial deletion of IS 256 in the left-hand flanking extremity of Tn 4001 . Structure III lacked both the right- and left-hand flanking extremities of Tn 4001 . One of the wild-type strains carried the Gm r determinant both on the chromosome, as a Tn 4001 -like α element, and on a conjugative plasmid, as a Tn 4001 -truncated type I structure.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference25 articles.

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