Affiliation:
1. Laboratoire des Staphylocoques et des Streptocoques, Institut Pasteur,1 and
2. Laboratoire de Microbiologie, Hôpital Broussais,2 Paris, France
Abstract
ABSTRACT
Of 24 high-level gentamicin-resistant clinical isolates of
Enterococcus faecalis
, 20 carried gentamicin resistance (Gm
r
) plasmids. The plasmids ranged from 65.0 to 80.0 kb in size. Three of these plasmids were nonconjugative, and 17 transferred by conjugation to an
E. faecalis
recipient at low frequency (10
−5
to 10
−6
transconjugants per donor). The remaining four strains had a nonconjugative chromosomal Gm
r
determinant. On the basis of restriction enzyme and DNA-DNA hybridization profiles, Tn
4001
-like α elements were located on the chromosome and three types of Tn
4001
-truncated structures, I, II, and III, were found to be carried by the Gm
r
plasmids. Structure I lacked IS
256
in the right-hand flanking extremity of Tn
4001
. Structure II was the same as structure I except that it also had a partial deletion of IS
256
in the left-hand flanking extremity of Tn
4001
. Structure III lacked both the right- and left-hand flanking extremities of Tn
4001
. One of the wild-type strains carried the Gm
r
determinant both on the chromosome, as a Tn
4001
-like α element, and on a conjugative plasmid, as a Tn
4001
-truncated type I structure.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
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