Reconstitution of Glyphosate Resistance from a Split 5-Enolpyruvyl Shikimate-3-Phosphate Synthase Gene in Escherichia coli and Transgenic Tobacco-Reference-Cited by-同舟云学术

Reconstitution of Glyphosate Resistance from a Split 5-Enolpyruvyl Shikimate-3-Phosphate Synthase Gene in Escherichia coli and Transgenic Tobacco

Published:2007-12-15 Issue:24 Volume:73 Page:7997-8000
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Dun Bao-Qing¹²,Wang Xu-Jing¹,Lu Wei¹,Zhao Zhong-Lin¹³,Hou Song-Na¹,Zhang Bao-Ming²,Li Gui-Ying²,Evans Thomas C.⁴,Xu Ming-Qun⁴,Lin Min¹

Affiliation:

1. Biotechnology Research Institute, Chinese Academy of Agriculture Sciences, Beijing 100081, China

2. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agriculture Sciences, Beijing 100081, China

3. College of Biological Sciences, China Agricultural University, Beijing 100094, China

4. New England Biolabs, Inc., Ipswich, Massachusetts 01938

Abstract

ABSTRACT A highly N -phosphonomethylglycine (glyphosate)-resistant Pseudomonas fluorescens G2 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) was mapped to identify potential split sites using a transposon-based linker-scanning procedure. Intein-mediated protein complementation was used to reconstitute glyphosate resistance from the genetically divided G2 EPSPS gene in Escherichia coli strain ER2799 and transgenic tobacco.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00956-07

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