Thymine at —5 Is Crucial for cpc Promoter Activity of Synechocystis sp. Strain PCC 6714-Reference-Cited by-同舟云学术

Thymine at —5 Is Crucial for cpc Promoter Activity of Synechocystis sp. Strain PCC 6714

Published:2003-11 Issue:21 Volume:185 Page:6477-6480
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Imashimizu Masahiko¹,Fujiwara Shoko¹²,Tanigawa Ryohei³,Tanaka Kan³,Hirokawa Takatsugu⁴,Nakajima Yuji⁵,Higo Junichi¹,Tsuzuki Mikio¹²

Affiliation:

1. School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji 192-0392

2. CREST, Japan

3. Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, Tokyo 113-0032

4. Computational Biology Research Center, National Institute of Advanced Industrial Science and Technology, Koutou-ku, Tokyo 135-0064

5. Advanced Technology Research Center, Mitsubishi Heavy Industries, Ltd., Kanazawa-ku, Yokohama 236-8515, Japan

Abstract

ABSTRACT The levels of transcripts of the cpc operon were highly reduced in a PD-1 mutant of cyanobacterium Synechocystis sp. strain PCC 6714. This was due to a substitution of C for T that occurred at 5 bp upstream of the transcription initiation site of the cpc operon. Any substitution for T at the −5 position drastically reduced both in vivo and in vitro promoter activity in cyanobacterium Synechococcus sp. strain PCC 7942 but not the in vivo activity in Escherichia coli . This suggests that the requirement of −5T appears to be specific for a cyanobacterial RNA polymerase-promoter combination.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.185.21.6477-6480.2003

Reference24 articles.

1. Genes for phycocyanin subunits in Synechocystis sp. strain PCC 6701 and assembly mutant UV16

2. Andersson, C. R., N. F. Tsinoremas, J. Shelton, N. V. Lebedeva, J. Yarrow, H. Min, and S. S. Golden. 2000. Application of bioluminescence to the study of circadian rhythms in cyanobacteria. Methods Enzymol.305:527-542.

3. Belknap, W. R., and R. Haselkorn. 1987. Cloning and light regulation of expression of the phycocyanin operon of the cyanobacterium Anabaena.EMBO J.6:871-884.

4. Conley, P. B., P. G. Lemaux, and A. Grossman. 1988. Molecular characterization and evolution of sequences encoding light-harvesting components in the chromatically adapting cyanobacterium Fremyella diplosiphon.J. Mol. Biol.199:447-465.

5. Cramer, P., D. A. Bushnell, and R. D. Kornberg. 2001. Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution. Science292:1863-1876.

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