Structure of Haloacetate-Catabolic IncP-1β Plasmid pUO1 and Genetic Mobility of Its Residing Haloacetate-Catabolic Transposon-Reference-Cited by-同舟云学术

Structure of Haloacetate-Catabolic IncP-1β Plasmid pUO1 and Genetic Mobility of Its Residing Haloacetate-Catabolic Transposon

Published:2003-11-15 Issue:22 Volume:185 Page:6741-6745
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Sota Masahiro¹²,Kawasaki Haruhiko³,Tsuda Masataka²

Affiliation:

1. Department of Environmental Simulation, Institute for Environmental Sciences, Rokkasho, Aomori 039-3212

2. Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai 980-8577

3. Department of Applied Biochemistry, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan

Abstract

ABSTRACT The self-transmissible plasmid pUO1 from Delftia acidovorans strain B carries two haloacetate-catabolic transposons, Tn Had1 and Tn Had2 , and the mer genes for resistance to mercury. The complete 67,066-bp sequence of pUO1 revealed that the mer genes were also carried by two Tn 402 /Tn 5053 -like transposons, Tn 4671 and Tn 4672 , and that the pUO1 backbone regions shared 99% identity to those of the archetype IncP-1β plasmid R751. Comparison of pUO1 with three other IncP-1β plasmids illustrated the importance of transposon insertion in the diversity and evolution of this group of plasmids. Mutational analysis of the four outermost residues in the inverted repeats (IRs) of Tn Had2 , a Tn 21 -related transposon, revealed a crucial role of the second residue of its IRs in transposition.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.185.22.6741-6745.2003

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