Identification of enterotoxigenic Escherichia coli by colony hybridization with nonradioactive digoxigenin-labeled DNA probes-Reference-Cited by-同舟云学术

Identification of enterotoxigenic Escherichia coli by colony hybridization with nonradioactive digoxigenin-labeled DNA probes

Published:1990-06 Issue:6 Volume:28 Page:1465-1468
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Riley L K¹,Caffrey C J¹

Affiliation:

1. Department of Veterinary Pathology, University of Missouri, Columbia 65211.

Abstract

Enterotoxigenic Escherichia coli (ETEC) strains were readily identified in pure and mixed cultures with nonradioactive, digoxigenin-labeled DNA probes coding for heat-labile (LTI) and heat-stable (STaI, STaII, and STb) enterotoxins. Digoxigenin-labeled ETEC fragments were more sensitive than and exhibited less nonspecific background contamination than biotinylated ETEC probes.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.28.6.1465-1468.1990

Reference18 articles.

1. Detection of enterotoxigenic Escherichia col by dot blot hybridization with biotinylated DNA probes;Bialkowska-Hobrzanska H.;J. Clin. Microbiol.,1987

2. Cistrons encoding Escherichia coli heat-labile toxin;Dallas W. S.;J. Bacteriol.,1979

3. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu;Dean A. G.;J. Infect. Dis.,1972

4. Detection of enterotoxigenic Escherichia coli by colony hybridization with biotinylated enterotoxin probes;Kirii Y.;J. Clin. Microbiol.,1987

5. Cell free synthesis of enterotoxin of Escherichia coli from a cloned gene;Lathe R.;Nature (London),1980

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