Detection and characterization of fecal verotoxin-producing Escherichia coli from healthy cattle

Author:

Montenegro M A1,Bülte M1,Trumpf T1,Aleksić S1,Reuter G1,Bulling E1,Helmuth R1

Affiliation:

1. Institute for Veterinary Medicine (Robert von Ostertag Institute), Federal Health Office, Berlin, Federal Republic of Germany.

Abstract

Verotoxin-producing Escherichia coli isolates from feces of healthy cattle were identified by DNA hybridization with verotoxin 1- and verotoxin 2-specific gene probes. Among 259 animals investigated, 28 (10.8%) were found to carry verotoxin-producing E. coli strains. Characterization of the verotoxin-producing isolates revealed a heterogeneous population in terms of serotype and toxin type. Nearly 40% of the strains belonged to serogroups known to be pathogenic for humans, i.e., O22, O39, O82, O91, O113, O116, O126, and O136. Two isolates from different bulls were identified as serotype O157:H7. Results obtained in this study indicate that cattle may be an important source of verotoxigenic E. coli involved in human disease.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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