Sequential Phosphorylation of Hepatitis C Virus NS5A Protein Requires the ATP-Binding Domain of NS3 Helicase-Reference-Cited by-同舟云学术

Sequential Phosphorylation of Hepatitis C Virus NS5A Protein Requires the ATP-Binding Domain of NS3 Helicase

Published:2022-04-13 Issue:7 Volume:96 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Yu Chun-Chiao¹,Lin Pei-Chen¹,Chiang Cho-Han²,Jen Shu-Tang¹,Lai Yen-Ling¹,Hsu Shih-Chin¹,Lo Lee-Chiang³,Lin Jing-Jer¹,Chan Nei-Li¹,Yu Ming-Jiun¹^ORCID

Affiliation:

1. Institute of Biochemistry and Molecular Biology, National Taiwan University, Taipei, Taiwan

2. Department of Medicine, National Taiwan University, Taipei, Taiwan

3. Department of Chemistry, National Taiwan University, Taipei, Taiwan

Abstract

For more than 20 years, NS3 was known to participate in NS5A sequential phosphorylation. In the present study, we show for the first time that the ATP-binding domain of NS3 is involved in NS5A phosphorylation. In vitro assays showed that casein kinase Iα is a very potent kinase responsible for NS5A phosphorylation at serines 225, 232, and 235.

Funder

National Health Research Institutes

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.00107-22

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