Molecular Confirmation of Human Immunodeficiency Virus (HIV) Type 2 in HIV-Seropositive Subjects in South India-Reference-Cited by-同舟云学术

Molecular Confirmation of Human Immunodeficiency Virus (HIV) Type 2 in HIV-Seropositive Subjects in South India

Published:2000-11 Issue:6 Volume:7 Page:987-989
ISSN:1071-412X
Container-title:Clinical Diagnostic Laboratory Immunology
language:en
Short-container-title:Clin Diagn Lab Immunol

Author:

Kannangai R.¹,Ramalingam S.¹,Prakash K. J.¹,Abraham O. C.²,George R.³,Castillo R. C.⁴,Schwartz D. H.⁴,Jesudason M. V.⁵,Sridharan G.¹

Affiliation:

1. Departments of Clinical Virology,1

2. Internal Medicine Unit I,2

3. Dermatology,3and

4. Department of Molecular Microbiology and Immunology, The Johns Hopkins School of Hygiene and Public Health, Baltimore, Maryland 212054

5. Clinical Microbiology,5 Christian Medical College Hospital, Vellore, India, 632004, and

Abstract

ABSTRACT Nested PCRs for human immunodeficiency virus type 1 (HIV-1) and HIV-2 were compared with immunoblot test results. Twelve of 13 immunoblot-positive HIV-2 samples were positive by PCR. There were five INNO-LIA (Innogenetics, Zwijnaarde, Belgium) and/or HIVBLOT 2.2 (Genelabs, Singapore) samples that tested positive for dual infection. HIV-1 PCR was positive in all samples, while HIV-2 PCR was positive in two and RIBA (Chiron Corporation, San Diego, Calif.) was positive for HIV-2 in three samples. Thus the prevalence of HIV-2 is accurately estimated by the use of immunoblotting, but that of HIV-1 and -2 dual infection may be overestimated.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CDLI.7.6.987-989.2000

Reference22 articles.

1. The detection of HIV-2 infection in southern India.;Babu P. G.;Indian J. Med. Res.,1993

2. Serological evidence of virus related to simian T-lymphotrophic retrovirus III in residents of West Africa.;Brain F.;Lancet,1985

3. Highly Sensitive Method for Amplification of Human Immunodeficiency Virus Type 2 DNA

4. Genetic subtyping of human immunodeficiency virus using heteroduplex mobility assay.;Delwart E. L.;PCR Methods Appl.,1995

5. Erlich H. A. Bugawan T. L. HLA DNA typing PCR protocols: a guide to methods and application. Innis M. A. Elfand D. H. Sninsky J. J. White T. J. 1990 261 271 Academic Press San Diego Calif

Cited by 19 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. The development, evaluation, performance, and validation of micro-PCR and extractor for the quantification of HIV-1 &-2 RNA;Scientific Reports;2024-04-15

2. HIV-1/2 differentiation in a South African public laboratory;Southern African Journal of HIV Medicine;2021-03-12

3. Genetic Characterization of Full-Length HIV-2 Long Terminal Repeat Sequences: Identification of Rare Promoter Variants;AIDS Research and Human Retroviruses;2020-06-01

4. Male Circumcision and the Epidemic Emergence of HIV-2 in West Africa;PLOS ONE;2016-12-07

5. Human Immunodeficiency Virus;Manual of Commercial Methods in Clinical Microbiology;2014-04-09