Abstract
A qualitative micromethod (IDS Rapid NH system) employing conventional and single-substrate enzyme tests was developed for the biochemical characterization of Neisseria spp., Haemophilus spp., and other gram-negative species. A total of over 140 dehydrated, miniaturized biochemical tests were investigated for their ability to distinguish species. Computer-assisted test selection and pair separation analysis of the data allowed the selection of 11 4-h tests that would identify Haemophilus and Neisseria spp. implicated as etiological agents as well as differentiate them from other Neisseria spp., Moraxella spp., Branhamella catarrhalis, Centers for Disease Control M groups, and Kingella spp. The final test configuration included modified glucose, sucrose, galactosidase, nitrate, phosphatase, resazurin reduction, and two arylamidase tests. In addition, indole, urea, and ornithine decarboxylase tests were included to biochemically type strains of Haemophilus influenzae and Haemophilus parainfluenzae.
Publisher
American Society for Microbiology
Reference29 articles.
1. Biochemical characteristics of Haemophillus influenzae in relationship to source of isolation and antibiotic resistance;Albritton W. L.;J. Clin. Microbiol.,1978
2. Use of the Minitek system for biotyping Haemophiluls species;Back A. E.;J. Clin. Microbiol.,1978
3. Modification of the rapid fermentation test for Neisseria gonorrhoeae;Brown W. J.;AppI. Microbiol.,1974
4. Some growth requirements of Haemophilus influenzae and Haemophilus pertussis;Brumfitt W.;J. Pathol. Bacteriol.,1959
5. Hydrolases of Neisseria gonorrhoeae;Chen K. C. S.;J. Biol. Chem.,1980
Cited by
10 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献