Accuracy of Different Bioinformatics Methods in Detecting Antibiotic Resistance and Virulence Factors from Staphylococcus aureus Whole-Genome Sequences

Author:

Mason Amy1,Foster Dona12,Bradley Phelim3,Golubchik Tanya1,Doumith Michel4,Gordon N. Claire1ORCID,Pichon Bruno4,Iqbal Zamin3,Staves Peter4,Crook Derrick1562,Walker A. Sarah162,Kearns Angela46,Peto Tim162

Affiliation:

1. Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom

2. NIHR Oxford Biomedical Research Centre, University of Oxford, Oxford, United Kingdom

3. Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom

4. Staphylococcus Reference Service, National Infection Service, Public Health England, London, United Kingdom

5. National Infection Service, Public Health England, London, United Kingdom

6. The National Institute for Health Research (NIHR) Health Protection Research Unit in Healthcare Associated Infections and Antimicrobial Resistance, University of Oxford, Oxford, United Kingdom

Abstract

In principle, whole-genome sequencing (WGS) can predict phenotypic resistance directly from a genotype, replacing laboratory-based tests. However, the contribution of different bioinformatics methods to genotype-phenotype discrepancies has not been systematically explored to date.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference50 articles.

1. Staphylococcus aureusInfections

2. Public Health England. 2016. Annual epidemiological commentary: mandatory MRSA, MSSA and E. coli bacteraemia and C. difficile infection data 2015/16. Public Health England, London, United Kingdom. https://www.gov.uk/government/uploads/system/uploads/attachment_data/file/535635/AEC_final.pdf.

3. The Impact of Methicillin Resistance in Staphylococcus aureus Bacteremia on Patient Outcomes: Mortality, Length of Stay, and Hospital Charges

4. Randomized Trial of Rapid Multiplex Polymerase Chain Reaction–Based Blood Culture Identification and Susceptibility Testing

5. A novel universal DNA labeling and amplification system for rapid microarray-based detection of 117 antibiotic resistance genes in Gram-positive bacteria

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3