Slicing through the challenge of maintaining <i>Pneumocystis</i> in the laboratory-Reference-Cited by-同舟云学术

Slicing through the challenge of maintaining Pneumocystis in the laboratory

Published:2024-03-13 Issue:3 Volume:15 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Nev Olga A.¹²^ORCID,Duvenage Lucian²³⁴,Brown Alistair J. P.²^ORCID,Dangarembizi Rachael²³⁵⁶,Hoving Jennifer Claire²³⁴

Affiliation:

1. Biosciences and Living Systems Institute, University of Exeter, Exeter, United Kingdom

2. Medical Research Council Centre for Medical Mycology, University of Exeter, Exeter, United Kingdom

3. CMM AFRICA Medical Mycology Research Unit, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa

4. Department of Pathology, Division of Immunology, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa

5. Department of Human Biology, Division of Physiological Sciences, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa

6. Neuroscience Institute, Faculty of Health Sciences, University of Cape Town, Groote Schuur Hospital, Cape Town, South Africa

Abstract

ABSTRACT Pneumocystis jirovecii is a major fungal pathogen of humans that causes life-threatening lung infections in immunocompromised individuals. Despite its huge global impact upon human health, our understanding of the pathobiology of this deadly fungus remains extremely limited, largely because it is not yet possible to cultivate Pneumocystis in vitro, independently of the host. However, a recent paper by Munyonho et al. offers a major step forward (F. T. Munyonho, R. D. Clark, D. Lin, M. S. Khatun, et al., 2023, mBio 15:e01464-23, https://doi.org/10.1128/mbio.01464-23 ). They show that it is possible to maintain both the trophozoite and cyst forms of the mouse pathogen, Pneumocystis murina, in precision-cut lung slices for several weeks. Furthermore, they demonstrate that this offers the exciting opportunity to examine potential virulence factors such as possible biofilm formation as well as antifungal drug responses in the lung.

Funder

UKRI | Medical Research Council

Carnegie Corporation of New York

Wellcome Trust

UK Research and Innovation

Publisher

American Society for Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mbio.03277-23

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