Affiliation:
1. Program in Molecular Biology, Sloan Kettering Cancer Center, New York, New York 10021, USA.
Abstract
The HIR/HPC (histone regulation/histone periodic control) negative regulators play important roles in the transcription of six of the eight core histone genes during the Saccharomyces cerevisiae cell cycle. The phenotypes of hir1 and hir2 mutants suggested that the wild-type HIR1 and HIR2 genes encode transcriptional repressors that function in the absence of direct DNA binding. When Hir1p and Hir2p were artificially tethered to yeast promoters, each protein repressed transcription, suggesting that they represent a new class of transcriptional corepressors. The two proteins might function as a complex in vivo: Hir2p required both Hir1p and another Hir protein, Hir3p, to repress transcription when it was tethered to an HTA1-lacZ reporter gene, and Hir1p and Hir2p could be coimmunoprecipitated from yeast cell extracts. Tethered Hir1p also directed the periodic transcription of the HTA1 gene and repressed HTA1 transcription in response to two cell cycle regulatory signals. Thus, it represents the first example of a transcriptional corepressor with a direct role in cell cycle-regulated transcription.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Reference65 articles.
1. Identification of a DNA binding factor involved in cell-cycle control of the yeast HO gene;Andrews B. J.;Cell,1989
2. Modifiers of position effect are shared between telomeric and silent mating-type loci in S. cerevisiae;Aparicio O. M.;Cell.,1991
3. Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. G. Seidman J. A. Smith and K. Struhl (ed.). 1989. Current protocols in molecular biology. Wiley Interscience and Green Publishing Associates New York N.Y.
4. Start-specific transcription in yeast;Breeden L.;Curr. Top. Microbiol. Immunol.,1996
5. Three independent forms of regulation affect expression of HO, CLN1, and CLN2 during the cell cycle of Saccharomyces cerevisiae;Breeden L.;Genetics,1994
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