Nocardia sp. Carboxylic Acid Reductase: Cloning, Expression, and Characterization of a New Aldehyde Oxidoreductase Family-Reference-Cited by-同舟云学术

Nocardia sp. Carboxylic Acid Reductase: Cloning, Expression, and Characterization of a New Aldehyde Oxidoreductase Family

Published:2004-03 Issue:3 Volume:70 Page:1874-1881
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

He Aimin¹,Li Tao²,Daniels Lacy³,Fotheringham Ian⁴,Rosazza John P. N.¹

Affiliation:

1. Division of Medicinal and Natural Products Chemistry and Center for Biocatalysis and Bioprocessing, College of Pharmacy

2. Schering-Plough Corporation, Union, New Jersey 07083

3. Department of Microbiology, College of Medicine, University of Iowa, Iowa City, Iowa 52242

4. Department of Chemistry, Edinburgh University, Edinburgh, Scotland

Abstract

ABSTRACT We have cloned, sequenced, and expressed the gene for a unique ATP- and NADPH-dependent carboxylic acid reductase (CAR) from a Nocardia species that reduces carboxylic acids to their corresponding aldehydes. Recombinant CAR containing an N-terminal histidine affinity tag had K m values for benzoate, ATP, and NADPH that were similar to those for natural CAR, and recombinant CAR reduced benzoic, vanillic, and ferulic acids to their corresponding aldehydes. car is the first example of a new gene family encoding oxidoreductases with remote acyl adenylation and reductase sites.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.70.3.1874-1881.2004

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