Affiliation:
1. Department of Microbiology, Immunology and Preventive Medicine, Iowa State University, Ames 50011.
Abstract
The objective of this study was to develop a means of identifying and analyzing mycoplasma gene regulatory elements. Analysis of Acholeplasma oculi ISM1499 transcriptional sequences was accomplished using a promoterless lacZ reporter gene and an integrative vector strategy. Seven promoter-containing fragments were identified, levels of beta-galactosidase were determined, and transcriptional start sites were mapped.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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