Explanation for the Apparent Inefficiency of Reduced Nicotinamide Adenine Dinucleotide in Energizing Amino Acid Transport in Membrane Vesicles

Abstract

Lineweaver-Burk plots of reduced nicotinamide adenine dinucleotide (NADH) oxidation by membrane preparations from Bacillus subtilis are biphasic, with two K m values for NADH. The higher K m corresponds to the only K m observed for NADH oxidation by whole cells, whereas the lower K m corresponds to that observed with open cell envelopes. Membrane preparations apparently contain a small fraction of open or inverted vesicles which is responsible for the low K m reaction, whereas entry of NADH into the larger portion of closed, normally oriented vesicles is rate limiting and responsible for the high K m reaction. In contrast, the oxidation of l -α-glycerol-phosphate (glycerol-P) by membrane preparations shows only one K m that corresponds to that of glycerol-P oxidation by whole cells or lysates. Since glycerol-P dehydrogenase (NAD independent) has the same K m , this enzyme reaction rather than entry of glycerol-P into vesicles represents the rate-limiting step for glycerol-phosphate oxidation. The K m for amino acid uptake by vesicles in the presence of NADH corresponds to the high K m for NADH oxidation, indicating that NADH energizes transport only if it enters closed, normally oriented vesicles. Studies with rotenone and proteolytic enzymes support this interpretation. The apparent efficiency of NADH in energizing uptake seems to be lower than that of glycerol-P because, under the experimental conditions usually employed, open or inverted vesicles that do not participate in amino acid uptake are responsible for the major portion of NADH oxidation. When the results are corrected for this effect, the efficiency of NADH is essentially the same as that of l -α-glycerol-P.