Affiliation:
1. Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113, 1 and
2. Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Saitama 332, 2 Japan
Abstract
ABSTRACT
The nuclear vitamin D receptor (VDR) is a member of a nuclear receptor superfamily and acts as a ligand-dependent transcription factor. A family of cotranscriptional activators (SRC-1, TIF2, and AIB-1) interacts with and activates the transactivation function of nuclear receptors in a ligand-dependent way. We examined interaction of VDR with these coactivators that was induced by several vitamin D analogs, since they exert differential subsets of the biological action of vitamin D through unknown mechanisms. Unlike other vitamin D analogs tested, OCT (22-
oxa
-1α,25-dihydroxyvitamin D
3
) induced interaction of VDR with TIF2 but not with SRC-1 or AIB-1. Consistent with these interactions, only TIF2 was able to potentiate the transactivation function of VDR bound to OCT. Thus, the present findings suggest that the structure of VDR is altered in a vitamin D analog-specific way, resulting in selective interactions of VDR with coactivators. Such selective interaction of coactivators with VDR may specify the array of biological actions of a vitamin D analog like OCT, possibly through activating a particular set of target gene promoters.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Reference53 articles.
1. Modulation of cell growth, differentiation, and production of interleukin-3 by 1 alpha, 25-dihydroxyvitamin D3 in the murine myelomonocytic leukemia cell line WEHI-3;Abe J.;Cancer Res.,1986
2. A novel vitamin D3 analog, 22-oxa-1,25-dihydroxyvitamin D3, inhibits the growth of human breast cancer in vitro and in vivo without causing hypercalcemia;Abe J.;Endocrinology,1991
3. AIB1, a steroid receptor coactivator amplified in breast and ovarian cancer;Anzick S. L.;Science,1997
4. Steroid hormone receptors: many actors in search of a plot;Beato M.;Cell,1995
5. Vitamin D, calcium, and epidermal differentiation;Bikle D. D.;Endocr. Rev.,1993
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